Composite

Part:BBa_K2549022:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-09)


aCD19-mN1c-GV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 534
    Illegal BsaI site found at 1928
    Illegal SapI.rc site found at 1408


Design Notes

αCD19-mN1c-GV2 was assembled by joining αCD19 with a CD8α signal peptide and a Myc tag on its N terminal, mouse Notch1 core and GAL4-VP64 together using ClonExpress[1]. αCD19 with a CD8α signal peptide and a Myc tag on its N terminal fragment was synthesized by IDT (gBlock).


Source

This part was assembled by CD8α signal peptide (Part:BBa_K2549044), cMyc-tag (Part:BBa_K823036), αCD19 (Part:BBa_K2549005), mouse Notch1 core (Part:BBa_K2549006) and GAL4 DNA binding domain (Part:BBa_K801032), a linker (Part:BBa_K2549045) and VP64 transcription activator (Part:BBa_K1982012).


References

  1. ↑ http://www.vazymebiotech.com/products_detail/productId=195.html